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Before it can serve this function, two noncoding stretches of RNA introns must be removed from the mRNA precursor sequence, and the remaining three fragments exons joined in a linear manner. It is presumably this conformational difference that confers relative resistance to proteolytic degradation of infectious prions, and permits them to be distinguished from the normal PrPC in infected tissue. This prevents free rotation around the bond between the carbonyl carbon and the nitrogen of the peptide bond. This graph illustrates that myoglobin has a higher oxygen affinity at all pO 2 values than does hemoglobin. Carboxypeptidases are used in determining the C-terminal amino acid.
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